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lightcycler control kit dna  (Roche)


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    Structured Review

    Roche lightcycler control kit dna
    Lightcycler Control Kit Dna, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+control+kit+dna/LightCycler+480+System/pm41712434-108-16-20
    Average 99 stars, based on 6 article reviews
    lightcycler control kit dna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Control:

    Article Title: Significance of the quantitative measurement of the chr16: 51320015 integration site in hepatocytes of patients with chronic hepatitis B.
    Article Snippet: DNA was extracted from biopsy specimens using a QIAamp® DNA Mini kit (Qiagen, Hilden, Germany). .. The levels of intrahepatic (IH) covalently closed circular DNA (cccDNA) were measured using reverse transcription‐quantitative (RT‐q)PCR analysis, as described previously (8). β-globin DNA (housekeeping gene) was detected using a LightCycler® Control kit DNA (Roche Diagnostics GmbH, Mannheim, Germany) in order to count the cell number in the biopsies and calculate the number of copies/cell. ..

    Article Title: Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis: relevance to localization of TREK-2 channels in astrocytes after transient cerebral ischemia.
    Article Snippet: PCR amplifications were carried out in glass capillary tubes (Roche Diagnostics). .. Reverse transcription was performed at 55°C for 600 s, and amplification began with a 30 s denaturation step at 95°C followed by 45 cycles of annealing at 55°C for 10 s and extension at 72°C for 13 s. We optimized the results by performing a standard curve for the mRNA using the LightCycler Control Kit DNA (Roche Diagnostics, cat. no. 12158833001). .. The data were analyzed using LightCycler Software (Roche Molecular Biochemicals).

    Article Title: Impact of a naturally occurring hepatitis B virus genotype D-specific core-promoter mutation on viral replication.
    Article Snippet: .. Cell number was estimated by quantifying β- globin gene levels in extracted genomic DNA using the LightCycler Control Kit DNA (Roche Diagnostics GmbH, Mannheim, Germany), and the results were normalized to 1×104 cells. .. Transcription factor binding assay The activities of HNF1 and HNF3 were then assessed using the TransAM flexi Transcription Factor Assay Kit (Active Motif, Rixensart, Belgium) in accordance with the manufacturer’s instructions.

    Article Title: Impact of a naturally occurring hepatitis B virus genotype D-specific core-promoter mutation on viral replication
    Article Snippet: .. Cell number was estimated by quantifying β -globin gene levels in extracted genomic DNA using the LightCycler Control Kit DNA (Roche Diagnostics GmbH, Mannheim, Germany), and the results were normalized to 1×10 4 cells. .. The activities of HNF1 and HNF3 were then assessed using the TransAM flexi Transcription Factor Assay Kit (Active Motif, Rixensart, Belgium) in accordance with the manufacturer’s instructions.

    Article Title: Improved detection of Clostridium difficile in animals by using enrichment culture followed by LightCycler real-time PCR.
    Article Snippet: The performance of our previously published TaqMan real-time PCR (TMrtPCR) for the detection of Clostridium difficile directly from animal faeces was found to be inadequate due to TMrtPCR false negative results.. Therefore, we developed a new internally controlled LightCycler real-time PCR (LC rtPCR) capable of detecting variant strains in diarrhoeic and subclinical animals by using two hybridisation probes instead of one hydrolysis probe used in TMrtPCR.. While LC rtPCR did not provide better results than TMrtPCR, improved detection of C. difficile in samples with low number of bacteria was introduced, using a preenrichment step followed by LC rtPCR.

    Article Title: Uracil DNA glycosylase interacts with the p32 subunit of the replication protein A complex to modulate HIV-1 reverse transcription for optimal virus dissemination.
    Article Snippet: .. Total DNA was expressed as copy numbers per cell, with the DNA template normalized by β-globin gene amplification using a LightCycler control kit DNA (Roche). ..

    Reverse Transcription:

    Article Title: Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis: relevance to localization of TREK-2 channels in astrocytes after transient cerebral ischemia.
    Article Snippet: PCR amplifications were carried out in glass capillary tubes (Roche Diagnostics). .. Reverse transcription was performed at 55°C for 600 s, and amplification began with a 30 s denaturation step at 95°C followed by 45 cycles of annealing at 55°C for 10 s and extension at 72°C for 13 s. We optimized the results by performing a standard curve for the mRNA using the LightCycler Control Kit DNA (Roche Diagnostics, cat. no. 12158833001). .. The data were analyzed using LightCycler Software (Roche Molecular Biochemicals).

    Amplification:

    Article Title: Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis: relevance to localization of TREK-2 channels in astrocytes after transient cerebral ischemia.
    Article Snippet: PCR amplifications were carried out in glass capillary tubes (Roche Diagnostics). .. Reverse transcription was performed at 55°C for 600 s, and amplification began with a 30 s denaturation step at 95°C followed by 45 cycles of annealing at 55°C for 10 s and extension at 72°C for 13 s. We optimized the results by performing a standard curve for the mRNA using the LightCycler Control Kit DNA (Roche Diagnostics, cat. no. 12158833001). .. The data were analyzed using LightCycler Software (Roche Molecular Biochemicals).

    Article Title: Uracil DNA glycosylase interacts with the p32 subunit of the replication protein A complex to modulate HIV-1 reverse transcription for optimal virus dissemination.
    Article Snippet: .. Total DNA was expressed as copy numbers per cell, with the DNA template normalized by β-globin gene amplification using a LightCycler control kit DNA (Roche). ..

    Hybridization:

    Article Title: Human Papillomavirus Load in Eyebrow Hair Follicles and Risk of Cutaneous Squamous Cell Carcinoma
    Article Snippet: .. The amount of the single-copy gene beta-globin in each sample and the number of DNA copies of HPV types 5, 8, 15, 20, 23, 24, 36, and 38 was determined by quantitative PCRwith type-specific primers and probes in the hybridization probe format using the "LightCycler-Control Kit DNA" (Roche) and protocols described before. ..

    Plasmid Preparation:

    Article Title: Predictive value of intrahepatic hepatitis B virus covalently closed circular DNA and total DNA in patients with acute hepatitis B and patients with chronic hepatitis B receiving anti‑viral treatment.
    Article Snippet: Two forward primers, [CCC1, 5'-GCGGWCTCC CCGTCTGTGCC-3'; diaphanous-related formin 1 (DRF1), 5'-GTCTGTGCCTTCTCATCTGC-3'], and one reverse primer, (CCC2, 5'-GTC ATGCCCCAAAGCCACC-3'), were used for cccDNA amplification using the LightCycler® system (Roche Diagnostics, Mannheim, Germany) and SYBR Premix Ex Taq (Takara Bio Inc., Otsu, Japan). .. Serial dilutions of a plasmid containing an HBV monomer (pHBVEcoRI) served as a quantification standard. β-globin DNA (house keeping gene) was detected using the LightCycler® Control kit DNA (Roche Diagnostics) in order to count the cell number in the biopsies and calculate copies/cell. ..



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